HPTLC ATLAS

 

Botanical Information Forsythia suspensa (Thunb.) Vahl

Synonyms Forsythia fortunei Lindl.
Ligustrum suspensum Thunb.
Rangium suspensum (Thunb.) Ohwi
Forsythia sieboldii (Zabel) Dippel
Lilac perpensa Lam.
Syringa suspensa Thunb.
Common name (en) Weeping forsythia
Common name (de) Hänge-Forsythie
Common name (th) เหลี่ยงเคี้ยว
Common name (vi) Liên kiều
Common name (zh) 连翘

Sample preparation To 1 g of the powdered herbal drug add 10 mL of methanol, sonicate for 10 min and centrifuge. Use the supernatant as test solution
Mobile phase Toluene, acetone, ethyl acetate, formic acid, water 40:50:60:6:6 (v/v/v/v/v)
Derivatization Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min
System Suitability Test (SST) Under development
Specific marker(s) Under UV 366 nm, after derivatization
  • Forsythoside A: brown zone at RF ~ 0.12
  • Pinoresinol acetate: brown zone at RF ~ 0.65
HPTLC Chromatograms (different origin(s))
ATLAS documents ATLAS Method - Forsythia suspensa
Image comparison of Forsythia suspensa
visionCATS data (CAMAG software)

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