HPTLC Association

1.   What is the HPTLC Association?

The International Association for the Advancement of High Performance Thin Layer Chromatography (HPTLC Association) promotes the use of HPTLC in plant analysis and other analytical fields. The Association brings together representatives from academia, industry, research, regulatory and standard setting bodies.

2.   What is HPTLC?

HPTLC is the High-Performance version of Thin Layer Chromatography and a state-of-the art technique for plant analysis. It features significantly shorter developing times, lower solvent consumptions and improved resolution. Highly reproducible results and traceable records are achieved through a standardized methodology and the use of suitable instruments (typically controlled by software) for all steps of the analysis. A system suitability test is used to qualify results.

The stationary phase is a HPTLC glass plate or aluminum sheet coated with a uniform thin layer (typically 200 micron) of porous particles (2 – 10 μm) with an average particle size of 5 μm. The layer typically consists of silica gel with a pore size of 60 Angstroms, a polymeric binder and a so called fluorescence indicator (F254). The standard format of the plate is 20×10 cm.

The apparatus for HPTLC consist of

  • A device suitable for application of samples as bands providing control of dimension and position of the application as well as applied sample volume
  • A suitable chromatographic chamber (typically a twin trough chamber) providing control of saturation and developing distance
  • A device suitable for controlling the activity of the stationary phase via relative humidity
  • A device suitable for reproducible drying of the developed plate
  • Suitable devices for reagent transfer and heating as part of the derivatization procedure
  • A device suitable for electronic documentation of chromatograms under UV 254 nm, UV 366 nm, and white light
  • For quantitative determinations a densitometer or image evaluation software

SOP for HPTLC (pdf)

 

3.   What is the purpose of the HPTLC Association?

  • Develop and validate common standards and methods for identification of plants and their adulterants
  • Establish and maintain an “International Atlas of HPTLC Methods for Identification of Herbal Drugs (ATLAS)” as a recognized reference tool for quality control
  • Serve as the worldwide leading resource in information about and training in HPTLC to the scientific community
  • Support pharmacopoeias and similar organizations of individual countries or regions with expertise, guidance, methods and validation data

4.   What are the benefits for members of the HPTLC Association?

By providing their expertise in different fields of plant analysis, methods for identification, samples for peer review and facilities for training in local language members will gain prestige and global recognition for their contribution to the common cause of improved quality of medicinal plants. Members will benefit from new HPTLC methods, validation data, continued training and information about the latest HPTLC equipment and software.

5. How can I be a member?

The Board of Directors will evaluate the submitted applications and will decide at its own discretion on the admission. In addition to supporting the goals of the HPTLC Association and interest to actively contribute to the worldwide HPTLC community, a potential member is required to adhere to the Articles of Incorporation as well as to the Bylaws.

An application for membership to the HPTLC Association will be evaluated against the following criteria:

  • Demonstrated expertise in HPTLC
  • Proof of serious interest in HPTLC as defined by the Association
  • Capability to contribute to the mission of the Association