HPTLC ATLAS

 

Botanical Information Tinospora cordifolia (Willd.) Miers ex Hook.f.&Thoms. (under development)

Synonyms Menispermum cordifolium Willd.
Tinospora cordifolia var. congesta Mujaffar, Yasin & Solanki
Tinospora fosbergii Kundu
Common name (en) Heart-leaved moonseed
Common name (hi) गिलोय
Common name (id) Bratawali
Common name (th) บอระเพ็ด

Sample preparation Reflux 2 g of coarsly powdered sample with 25 ml of methanol for 15 min, cool and filter. Reflux the residue further with 2x25 ml of chloroform, cool and filter. Combine all the filtrates and concentrate under vacuum to 5 mL
Mobile phase chloroform, methanol 85:15 (v/v)
Derivatization examine under UV light of 254 nm and 366 nm, spray with anisaldehyde sulfuric acid reagent. Heat the plate at 110° for 10 min and examine in daylight.

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