HPTLC ATLAS

 

Botanical Information Anemarrhena asphodeloides Bunge (procuring samples)

Synonyms Terauchia anemarrhenifolia Nakai
Common name (en) Zhi mu
Common name (ja) ハナスゲ
Common name (ko) 지모
Common name (vi) Tri mẫu
Common name (zh) 知母

Sample preparation Weigh 0.5 g of the powdered sample and place it in a 50-mL conical flask, then add 25 mL of absolute ethanol. Shake the mixture for about 15 h. Sonicate (220 W) the mixture for 40 min. Filter and transfer 10 mL of filtrate to a 50-mL round-bottomed flask. Evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Add 11 mL of 1.1 M hydrochloric acid. Reflux the solution for 2 h. Cool in an ice bath. Add dropwise about 1 mL of sodium hydroxide solution (40%, w/v) with shaking. Transfer the solution to a separating funnel. Extract for three times each with 50 mL of dichloromethane. Combine the extracts. Evaporate the solvent to dryness at reduced pressure in a rotary evaporator. Dissolve the residue in 4 mL of dichloromethane
Mobile phase Ethyl acetate, cyclohexane 1:4 (v/v)
Derivatization Spray with Anisaldehyde reagent, and heat the plate at 110°C until the spots or bands become visible

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