Botanical Information Plantago depressa Willd. (procuring samples)
| Synonyms | Plantago asiatica var. decumbens Turcz. |
| Plantago depressa f. glaberrima Kom. | |
| Plantago depressa f. magnibracteata Tosh.Tanaka & T.K.Zheng | |
| Plantago depressa f. minor Kom. | |
| Plantago depressa var. eudepressa Ganesch. | |
| Plantago paludosa Turcz. ex Ledeb. | |
| Plantago sibirica Georgi Beschr. | |
| Plantago sibirica Poir. | |
| Plantago tibetica Hook.f. & Thomson |
| Sample preparation | Weigh 2.5 g of the powdered sample and place it in a 50-mL centrifuge tube, then add 10 mL of methanol (60%). Sonicate (350 W) the mixture for 15 min. Centrifuge at about 4000 × g for 5 min. Filter through a 0.45-µm nylon filter |
| Mobile phase | Ethyl acetate, ethanol, formic acid, water 18:3:1.5:1 (v/v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Add 1.0 g of powdered sample to 10 mL of methanol, ultrasonicate for 30 min, filter and use the filtrate |
| Mobile phase | Ethyl acetate, methanol, formic acid, water 18:3:1.5:1 (v/v/v/v) |
| Derivatization | No derivatization |
| Sample preparation | Add 2.0 g of powdered sample to 10 mL of ethanol, heat under reflux for 30 min, cool, filter and make up the filtrate to 10 mL |
| Mobile phase | n-butanol, acetic acid, water 7:1:2 (v/v/v) |
| Derivatization | Spray with 2,4-dinitrophenylhydrazine and heat at 105°C for 2 min |