Botanical Information Eleutherococcus nodiflorus (Dunn) S.Y.Hu (under development)
| Synonyms | Acanthopanax gracilistylus W.W.Sm. |
| Acanthopanax gracilistylus var. major G.Hoo | |
| Acanthopanax gracilistylus var. trifoliolatus C.B.Shang | |
| Acanthopanax gracilistylus var. villosulus (Harms) H.L.Li | |
| Acanthopanax hondae Matsuda | |
| Acanthopanax hondae var. armatus Nakai | |
| Acanthopanax hondae var. inermis Nakai | |
| Acanthopanax nodiflorus Dunn | |
| Acanthopanax spinosus Pavol. | |
| Acanthopanax villosulus Harms | |
| Aralia scandens Poir. | |
| Eleutherococcus gracilistylus (W.W.Sm.) S.Y.Hu | |
| Eleutherococcus gracilistylus var. major (G.Hoo) H.Ohashi | |
| Eleutherococcus gracilistylus var. nodiflorus (Dunn) H.Ohashi | |
| Eleutherococcus gracilistylus var. trifoliolatus (C.B.Shang) H.Ohashi | |
| Eleutherococcus gracilistylus var. villosulus (Harms) Q.S.Wang | |
| Eleutherococcus villosulus (Harms) S.Y.Hu | |
| Hedera scandens (Poir.) DC. | |
| Common name (zh) | 刺五加 |
| Sample preparation | To 0.2 g of the powder, add 10 mL of dichloromethane, ultrasonicate for 30 min, and filter. Evaporate the filtrate to dryness, dissolve the residue in 1 mL of dichloromethane as the test solution |
| Mobile phase | Petroleum ether (60-90°C) , ethyl acetate, formic acid 10:3:0.1 (v/v/v), on HPTLC silica gel Nano-Durasil-20 MN |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear |
| HPTLC Chromatograms |
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| Sample preparation | To 0.3 g of the powdered herbal drug add 3 mL of methanol, heat in a water-bath at 60 °C for 1 min and filter |
| Mobile phase | Ethyl acetate, methylene chloride 2:98 (v/v) |
| Derivatization | Treat with anisaldehyde solution, heat at 105 °C for 5 min |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | Weigh 3.0 g of the powdered sample and place it in a 25-mL conical flask, then add 10 mL of ethanol (70%). Sonicate (150 W) the mixture for 10 min. Filter the mixture |
| Mobile phase | Dichloromethane, methanol, water 10:2:0.1 (v/v/v) |
| Derivatization | No derivatization |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| Sample preparation | Weigh 1 g of pulverized drug, add 10 mL of methanol, shake thoroughly, filter and evaporate the filtrate to dryness. Dissolve the residue in 1 mL of methanol and use this solution as the test solution |
| Mobile phase | Chloroform, methanol, water 70:30:4 (v/v/v) |
| Derivatization | Spray with sulfuric acid and heat at 105 °C |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
|
| HPTLC Chromatograms |
|
| Sample preparation | To 0.2 g of the powder, add 10 mL of dichloromethane, ultrasonicate for 30 min, and filter. Evaporate the filtrate to dryness, dissolve the residue in 1 mL of dichloromethane as the test solution |
| Mobile phase | Petroleum ether (60-90°C) , acetone, isopropanol, formic acid 12:2:0.5:0.1 (v/v/v/v) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots appear |
| HPTLC Chromatograms |
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