New HPTLC methods for Aloes

New HPTLC methods for the identification of Barbados Aloes (Aloe barbadensis, Aloe vera) and Cape Aloes (Aloe ferox) and the discrimination of both species according to Ph.Eur. have been uploaded to the methods list.

Your comments are welcome, contact us at


Project on Chaparral finalized and method released

The project on Chaparral leaf (Larrea tridentata) was successfully terminated and a new HPTLC method was released.

The HPTLC method for the identification of dried Chaparral leaf is based on the analysis of flavonoids and additionally the detection of nordihydroguaiaretic acid.

This project was performed by the HPTLC Association members Eike Reich, Anabella Rodas-Nelson, Roy Upton, William Georgian, and Dhananjay Meshram. The HPTLC method was developed by Anabella Rodas-Nelson (Starwest Botanicals) and Eike Reich (CAMAG Laboratory).

We thank all contributors for their support.

For questions and comments on the method, contact


New and updated HPTLC methods

Two new HPTLC methods for Benzoe sumatranus (Sumatra benzoin) and Benzoe tonkinensis (Siam benzoin) were released. Both methods feature the same method parameters as the previously published Harmonized method for resins, but include more samples and a more detailed description.

Three methods from the methods list were updated:


New HPTLC methods

The HPTLC Association presents two new HPTLC methods for the identification of Cannabis. One method is based on normal-phase separation on silica gel (pdf), the other method on C-18 modified reversed phase silica gel (pdf).

An HPTLC method for the identification of Vitex negundo leaf (pdf) according to the Herbal Medicines Compendium is published.

More methods.

Questions or comments?


Welcome new members

A warm welcome to our five new members!

  • Dr. Patricia Odumosu, Dept. of Pharmaceutical Chemistry, University of Jos, Nigeria
  • Mr. Robert Palmer, Herbs In A Bottle, Stamford, UK
  • Dr. Kerry Penman, Sanofi, Brisbane, Australia
  • Mr. Tomislav Sostaric, Chromatech Scientific P/L, Yangebup, Australia
  • Prof. Snezana Agatonovic-Kustrin, LaTrobe University, Victoria, Australia

We are looking forward to working with you.

The HPTLC Association has now 60 members, see our member list.

Are you interested in becoming a member of the HPTLC Association? See the checklist for applicants.


Three methods for Equisetum species updated

Three methods for Equisetum species (Equisetum palustre, Equisetum hyemale, Equisetum telmateia) have been updated with new HPTLC images. The methods are based on the Ph. Eur. monograph for Equisetum arvense. The mobile phase was not changed.

Download the new pdf files from the list of methods.


Methods for Common horsetail herb updated

The two methods (identification, test for adulteration) for Common horsetail herb (Equisetum arvense) have been updated with new HPTLC images which include reference solutions a and/or b and the result description according to Ph.Eur 8.0. The other method parameters such as the mobile phase remain unchanged.

Download the new pdf files from the list of methods.


Welcome new members

We are proud to welcome seven new members:

  • Dr. Yamilè Bernardo Otero, Härting S.A., Santiago de Chile, Chile
  • Dr. Karuna Shanker, CSIR-Central Institute of Medicinal and Aromatic plants (CIMAP), Lucknow, India
  • Dr. Aqib Zahoor, Herbion Pak. Pvt. Ltd, Karachi, Pakistan
  • Dr. Artur Likhanov, The National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • Ms. Ng Chiew Hoong, postgraduate student, Gelugor, Penang, Malaysia
  • Mr. Calen McKenzie, Vitality Works, Inc., Albuquerque, NM, USA
  • Dr. Ajai Prakash Gupta, Indian Institute of Integrative Medicine, Jammu, India

We are looking forward to working with you!


Projects on Phyllanthus amarus, Holy basil and Rosemary completed

HPTLC methods for the identification of Phyllanthus amarus leaf, Holy basil leaf (Ocimum sanctum) and Rosemary leaf (Rosmarinus officinalis) have been developed by members of the HPTLC Association. The methods have been submitted to USP and have recently been accepted.

Details on the projects. Download the new methods from the methods list.


New HPTLC methods

Many new HPTLC methods have been uploaded and several methods have been updated.

  • Harmonized method for identification of resins
  • Alpinia katsumadai (Katsumada galangal seed)
  • Eleutherococcus gracilistylus (Acanthopanax root bark)
  • Saposhnikovia divaricata (Saposhnikovia root)
  • Commiphora myrrha (Myrrh)
  • Panax quinquefolius (American ginseng root) - method USP
  • Panax ginseng (Asian ginseng root) - method USP
  • Panax notoginseng (Tienchi ginseng root) - method USP
  • Lagerstroemia speciosa (Banaba leaf)
  • Centella asiatica (Gotu kola herb)
  • Gymnema sylvestre (Gymnema leaf)
  • Coriandrum sativum fruit (Coriander fruit)
  • Coriandrum sativum essential oil (Coriander oil)
  • Cinnamomum cassia (Cassia cinnamon bark)
  • Cinnamomum verum (Cinnamon bark)
  • Ocimum sanctum (Holy basil leaf)
  • Schisandra chinensis (Schisandra chinensis fruit)
  • Rosmarinus officinalis (Rosemary leaf and hydrophilic extract) - method USP
  • Rhodiola rosea (Rhodiola rosea root)
  • Salvia miltiorrhiza (Chinese Salvia root) - method USP
  • Piper nigrum (Black pepper fruit) - method USP)