Projects on Phyllanthus amarus, Holy basil and Rosemary completed

HPTLC methods for the identification of Phyllanthus amarus leaf, Holy basil leaf (Ocimum sanctum) and Rosemary leaf (Rosmarinus officinalis) have been developed by members of the HPTLC Association. The methods have been submitted to USP and have recently been accepted.

Details on the projects. Download the new methods from the methods list.


New HPTLC methods

Many new HPTLC methods have been uploaded and several methods have been updated.

  • Harmonized method for identification of resins
  • Alpinia katsumadai (Katsumada galangal seed)
  • Eleutherococcus gracilistylus (Acanthopanax root bark)
  • Saposhnikovia divaricata (Saposhnikovia root)
  • Commiphora myrrha (Myrrh)
  • Panax quinquefolius (American ginseng root) - method USP
  • Panax ginseng (Asian ginseng root) - method USP
  • Panax notoginseng (Tienchi ginseng root) - method USP
  • Lagerstroemia speciosa (Banaba leaf)
  • Centella asiatica (Gotu kola herb)
  • Gymnema sylvestre (Gymnema leaf)
  • Coriandrum sativum fruit (Coriander fruit)
  • Coriandrum sativum essential oil (Coriander oil)
  • Cinnamomum cassia (Cassia cinnamon bark)
  • Cinnamomum verum (Cinnamon bark)
  • Ocimum sanctum (Holy basil leaf)
  • Schisandra chinensis (Schisandra chinensis fruit)
  • Rosmarinus officinalis (Rosemary leaf and hydrophilic extract) - method USP
  • Rhodiola rosea (Rhodiola rosea root)
  • Salvia miltiorrhiza (Chinese Salvia root) - method USP
  • Piper nigrum (Black pepper fruit) - method USP)


Successful HPTLC workshop

The recent workshop HPTLC for Popular Botanical Materials in a cGMP Compliant Environment was a success! The experts from HPTLC Association and AHPA provided the participants with unique insights on today's HPTLC. There was enough time for extensive hands-on laboratory work so that participants got to know the benefits and challenges of the technique.


HPTLC workshops with AHPA

From Aug. 31 to Sept. 2, 2015, the HPTLC Association and AHPA will hold two consecutive two-day workshops on HPTLC in Candler, NC, USA: HPTLC for Popular Botanical Materials in a cGMP Compliant Environment

Register now! Only few places left.



USP features two new chapters on HPTLC

The United States Pharmacopoeia recently published two important chapters on HPTLC in their First Supplement to USP 38–NF 33.

Chapter 1064 - Identification of Articles of Botanical Origin by High-Performance Thin-Layer Chromatography Procedure (pdf)

Chapter 203 - High-Performance Thin-Layer ChromatographyProcedure for Identification of Articles of Botanical Origin (pdf)



We are back!

Due to a shortage in staff we have been silent for a while. Now we are back and looking forward to hearing from you. Your input is welcome. Contact us at


Methods for black cohosh updated

The HPTLC method for identification of black cohosh (Cimicifuga racemosa) and the test for adulteration of black cohosh with other Cimicifuga species have been updated. See the methods list (sort by date of publication).


Welcome new members

The HPTLC Association welcomes five new members: Dr. Christopher Hobbs (UC Berkeley, Davis, CA, USA), Dr. Rosmarie Scheruebl (Regensburg, Germany), Dr. James Neal-Kababick (Flora Research Laboratories, Grants Pass, OR, USA), Mr. Rick Junk (Ortho Molecular Products, Stevens Point, WI, USA), and Dr. Dhananjay Meshram (Pioneer Pharmacy Degree College, Gujarat, India).

We are looking forward to working with you!


HPTLC methods updated

Four HPTLC methods have been updated with new images: Silybum marianum (Milk thistle fruit), Hypericum perforatum (St. John’s wort herb), Piper methysticum (Kava rhizome), and Zingiber officinale (Ginger rhizome).



Collection of HPTLC methods as pdf book

The book contains a selection of HPTLC identification methods featured on this website, an introduction by the Chair Method Review Comittee, the Association's definition and SOP of HPTLC, and an alphabetical table of contents.