HPTLC ATLAS

 

Botanical Information Hydrastis canadensis L.

Synonyms Hydrastis trifolia Raf.
Warneria canadensis Mill.
Common name (en) Goldenseal
Common name (ar) خاتم الذهب
Common name (de) Kanadische Orangenwurzel
Common name (es) Hidrastis
Common name (fr) Hydraste du Canada
Common name (it) Idraste
Common name (ko) 히드라스티스 카나덴시스
Common name (ru) Желтокорень
Common name (zh) 金印草

Sample preparation Sonicate 0.25 g of (finely) powdered Goldenseal root and rhizome in 4 mL of 80% methanol for 30 min. Withdraw the supernatant, and wash the residue twice with 2-mL aliquots of methanol. Combine the supernatant and rinse liquids in a 20-mL volumetric flask, and adjust with methanol to volume. Mix well and filter before use
Mobile phase Ethyl acetate, methanol, formic acid, and water 50:10:6:3 (v/v/v/v)
Derivatization No derivatization
System Suitability Test (SST) According to the monograph
Specific marker(s) Under UV 366 nm
  • USP Hydrastine RS: blue fluorescent zone at RF ~ 0.27
  • USP Berberine chloride RS: green fluorescent zone at RF ~ 0.46

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