HPTLC ATLAS

 

Botanical Information Berberis aristata DC. (under development)

Synonyms Berberis bussmul K.Koch ex Miq.
Berberis ceratophylla G.Don
Berberis chitria D.Don
Berberis chitria var. sikkimensis C.K.Schneid.
Berberis coccinea K.Koch
Berberis coerulescens G.Nicholson
Berberis elegans K.Koch
Berberis gracilis Lindl.
Berberis gracillima K.Koch
Berberis macrophylla K.Koch
Berberis serratifolia K.Koch
Berberis sikkimensis (C.K.Schneid.) Ahrendt
Berberis sikkimensis var. baileyi Ahrendt J. Linn.
Berberis sikkimensis var. glabramea Ahrendt
Berberis umbellata Lindl.
Berberis undulata K.Koch
Common name (en) Indian barberry
Common name (de) Begrannte Berberitze
Common name (hi) दारुहरिद्रा

Sample preparation Extract about 1 g, accurately weighed, powdered drug with 10 mL of methanol by heating on a water bath for 10 min with intermittent shaking. Filter and use the filtrate
Mobile phase n-propanol, formic acid (85%), water 9:01:0.9 (v/v/v)
Derivatization Spray with Dragendorff reagent
Sample preparation To 1 g of the coarsely powdered substance, add 40 mL of methanol, reflux for 15 minutes, cool and filter. Reflux the residue further for two times with 25 mL of methanol, cool and filter. Combine all the filtrates and concentrate under vacuum to 25 mL
Mobile phase Ethyl acetate, formic acid, acetic acid, water 80:10:10:20 (v/v/v/v/v)
Derivatization No derivatization
Sample preparation Shake 1 g of powdered substance with 10 mL of methanol for 10 to 15 min and filter. Wash the residue with 5 mL of methanol, add washing to the filtrate
Mobile phase n-butanol, acetic acid, water 70:10:20 (v/v/v)
Derivatization No derivatization

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