Botanical Information Zingiber officinale Roscoe
| Synonyms | Amomum zingiber L. |
| Amomum zinziba Hill | |
| Zingiber cholmondeleyi (F.M.Bailey) K. Schum. | |
| Zingiber missionis Wall. | |
| Zingiber sichuanense Z. Y. Zhu, S. L. Zhang & S. X. Chen | |
| Zingiber zingiber (L.) H. Karst. | |
| Common name (en) | Ginger |
| Common name (de) | Ingwer |
| Common name (fr) | Gingembre |
| Common name (ja) | ショウガ |
| Common name (ko) | 생강 |
| Common name (th) | ขิง |
| Common name (vi) | Gừng |
| Common name (zh) | 薑 |
| Sample preparation | Dilute 10 µL of the essential oil to be examined to 1 mL with toluene |
| Mobile phase | Toluene, ethyl acetate 95:5 (v/v) |
| Derivatization | Treat with anisaldehyde solution, heat at 100 °C for 3 min; examine immediatly in daylight |
| System Suitability Test (SST) | Under UV 254, prior to derivatization
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| Specific marker(s) | Under UV 366 nm, after derivatization
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| HPTLC Chromatograms |
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| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Toluene, ethyl acetate 3:1 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min. |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under UV 366 nm, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
Atlas method document of Zingiber officinale Image comparison of Zingiber officinale Image comparison of Zingiber officinale and related herbal drugs Selection of the ATLAS method of Zingiber officinale visionCATS data (CAMAG software) |
| Sample preparation | Alcoholic extract |
| Mobile phase | Benzene, ethyl acetate 9:1 (v/v) |
| Derivatization | iodine vapour |
| HPTLC Chromatograms |
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| Sample preparation | Alcoholic extract |
| Mobile phase | Benzene, ethyl acetate 9:1 (v/v) |
| Derivatization | Spray with a solution vanillin in sulfuric acid and heat at 105°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | To 1.0 g of the powdered herbal drug add 5 mL of methanol. Shake for 15 min and filter |
| Mobile phase | Hexane, ether 40:60 (v/v) |
| Derivatization | Spray with vanillin-sulfuric acid reagent and heat the plate for 10 min at 100-105°C |
| HPTLC Chromatograms |
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| Sample preparation | Reflux 1 g of the coarsely powdered substance with 25 mL of methanol for 15 minutes, cool and filter. Wash the residue with 10 mL of methanol. Combine all the filtrates and span style="color:red">concentrate to 10 mL |
| Mobile phase | Hexane, diethyl ether 30:70 (v/v) |
| Derivatization | Spray the plate with vanillin-sulfuric acid reagent and heat the plate for 5-10 minutes at 100°C |
| HPTLC Chromatograms |
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| Sample preparation | To 2 g of pulverized Ginger add 5 mL of diethyl ether, shake for 10 minutes, filter, and use the filtrate as the sample solution |
| Mobile phase | Ethyl acetate, hexane 1:1 (v/v) |
| Derivatization | Spray evenly the reagent (4-dimethylaminobenzaldehyde TS) on the plate, then heat the plate for 5 min at 105°C, and allow to cool. |
| HPTLC Chromatograms |
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| Sample preparation | Weight 2 g of pulverized Ginger, add 5 mL of acetone, shake for 3 min, filter and use the filtrate as the test solution. |
| Mobile phase | Hexane, acetone, acetic acid 10:7:1 (v/v/v) |
| Derivatization | Spray evenly the plate with 2,4-dinitrophenylhydrazine and heat at 105°C for 10 minutes |
| Sample preparation | Weigh about 0.5 g of Z. officinale dried rhizome powder in a 50 mL screw-capped conical flask and add 10 mL methanol. Sonicate in a water bath of 60°C for 30 min. Cool the mixture and allow the insoluble matter to settle. Filter the mixture and use the filtrate as the test solution |
| Mobile phase | Toluene, ethyl acetate 3:1 (v/v) |
| Derivatization | Spray with anisaldehyde solution |
| Sample preparation | To 1 g of the powder, add 20 mL of ethyl acetate. Sonicate for 10 minutes and filter. Use the filtrate as test solution |
| Mobile phase | Petroleum ether (60-90°C) , chloroform, ethyl acetate 2:1:1 (v/v/v) |
| Derivatization | Spray with vanillin-sulfuric acid reagent and heat the plate at 105°C to the spot clear. |
| HPTLC Chromatograms |
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| Sample preparation | Alcoholic extract |
| Mobile phase | n-hexane, diethyl ether 4:6 (v/v) |
| Derivatization | Iodine vapor |
| HPTLC Chromatograms |
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| Sample preparation | Alcoholic extract |
| Mobile phase | n-hexane, diethyl ether 4:6 (v/v) |
| Derivatization | Spray with a solution vanillin in sulfuric acid and heat at 105°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | Add 2.0 g of powdered sample to 10 mL of acetone, sonicate for 30 minutes, filter and use the filtrate |
| Mobile phase | Toluene, acetone 4:1 (v/v) |
| Derivatization | Spray with a solution vanillin in sulfuric acid and heat at 105°C for 2 minutes until the spots become visible |
| HPTLC Chromatograms |
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| Sample preparation | 1 g of powdered sample with 5 mL methanol, shaking 15 minutes and filtering |
| Mobile phase | Hexane, ether 4:6 (v/v) |
| Derivatization | Spray with a solution vanillin in sulfuric acid and heat at 105°C for 10 min |
| HPTLC Chromatograms |
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| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Toluene, ethyl acetate 3:1 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, heat at 100°C for 3 min, and examine. |
| System Suitability Test (SST) | According to the monograph |
| Specific marker(s) |
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| HPTLC Chromatograms |
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| ATLAS documents |
Zingiber officinale rhizome USP method |
| Sample preparation | 2 g of powder extract with 5 mL of acetone, shake for 3 min. Filtrate and use the filtrate as test solution |
| Mobile phase | n-hexane, acetone, acetic acid 7.5:2.5:4 drops (v/v/drops) |
| Derivatization | Spray with a solution vanillin in sulfuric acid and heat at 110°C until the colors appear |
| HPTLC Chromatograms |
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