HPTLC ATLAS

 

Botanical Information Piper nigrum L.

Synonyms Muldera multinervis MiQ.
Muldera wightina MiQ.
Piper aromaticum Lam.
Piper trioicum Roxb.
Common name (en) Black pepper
Common name (de) Pfeffer
Common name (fr) Poivre noir
Common name (hi) काली मिर्च
Common name (id) Lada
Common name (it) Pepe
Common name (ja) コショウ
Common name (ko) 후추
Common name (nl) Peper
Common name (pt) Pimenta-preta
Common name (ru) Перец чёрный
Common name (th) พริกไทย
Common name (vi) Hồ tiêu
Common name (zh) 黑胡椒

Sample preparation
Mobile phase
Derivatization
Sample preparation To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge
Mobile phase Cyclohexane, ethyl acetate 5:3 (v/v)
Derivatization Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min
System Suitability Test (SST) Under development
Specific marker(s) Under white light, after derivatization
  • Piperine: green zone RF ~ 0.16
  • Borneol: brown zoneRF ~ 0.48
HPTLC Chromatograms (different origin(s))
HPTLC Chromatograms (related drug(s))
ATLAS documents ATLAS Method document of Piper nigrum
Image comparison of Piper nigrum
Image comparison of Piper nigrum and related herbal drugs
Selection of the ATLAS method of Piper nigrum
visionCATS data (CAMAG)

back