HPTLC ATLAS

 

Botanical Information Piper retrofractum Vahl

Synonyms Piper chaba Hunter
Piper officinarum (Miq.) C. DC.
Chavica officinarum Miq.
Common name (en) Balinese long pepper
Common name (fr) Tiêu dôi

Sample preparation To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge
Mobile phase Cyclohexane, ethyl acetate 5:3 (v/v)
Derivatization Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min
System Suitability Test (SST) Under development
Specific marker(s) Under white light, after derivatization
  • Piperine: green zone RF ~ 0.16
  • Borneol: brown zoneRF ~ 0.48
HPTLC Chromatograms (different origin(s))
HPTLC Chromatograms (related drug(s))
ATLAS documents ATLAS Method document of Piper retrofractum
Image comparison of Piper retrofractum
Image comparison of Piper retrofractum and related herbal drugs
Selection of the ATLAS method of Piper retrofractum
visionCATS data (CAMAG software)
Sample preparation extract 4 g powdered drug with 100 mL of chloroform in a soxhlet apparatus for 4 hours. Filter and concentrate the extract under reduced pressure to 10 mL.
Mobile phase n-hexane, acetone 7:3 (v/v)
Derivatization No derivatization

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