Botanical Information Piper retrofractum Vahl
| Synonyms | Piper chaba Hunter |
| Piper officinarum (Miq.) C. DC. | |
| Chavica officinarum Miq. | |
| Common name (en) | Balinese long pepper |
| Common name (fr) | Tiêu dôi |
Related herbal drug(s)
Piper cubeba L. f.
Piper longum L.
Piper nigrum L.
Piper kadsura (Choisy) Ohwi (under development)
| Sample preparation | To 1.0 g of the powdered herbal drug add 10 mL of methanol. Sonicate for 10 min and centrifuge |
| Mobile phase | Cyclohexane, ethyl acetate 5:3 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| System Suitability Test (SST) | Under development |
| Specific marker(s) | Under white light, after derivatization
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| HPTLC Chromatograms |
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| HPTLC Chromatograms |
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| ATLAS documents |
Image comparison of Piper retrofractum ATLAS Method document of Piper retrofractum Image comparison of Piper retrofractum and related herbal drugs Selection of the ATLAS method of Piper retrofractum visionCATS data (CAMAG software) |
| Sample preparation | To 0.5 g of the powder add 5 mL of methanol, sonicate for 10 min, centrifuge and use the supernatant |
| Mobile phase | Ethyl acetate, cyclohexane 30:50 (v/v) |
| Derivatization | Derivatize with anisaldehyde reagent, and heat the plate at 100°C for 5 min |
| HPTLC Chromatograms |
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| Sample preparation | Weigh 0.5 g of the freshly powdered sample and place it in a 15-mL centrifuge tube wrapped in aluminum foil, then add 10 mL of methanol. Sonicate (140 W) the mixture for 30 min. Centrifuge at about 2800 × g for 10 min. Filter through a 0.45-µm nylon filter. Freshly prepare the test solution |
| Mobile phase | Methanol, water 9:1 (v/v) (HPTLC RP-18 F254 plate) |
| Derivatization | Spray with 10% sulfuric acid in ethanol and heat the plate at 105°C for 3 min |
| HPTLC Chromatograms |
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| Sample preparation | 4 g of powdered drug with 100 mL of chloroform in a soxhlet apparatus 4h. Filter and concentrate the extract under reduced pressure up to around 10 mL |
| Mobile phase | n-hexane, acetone 6.5:3.5 (v/v) |
| Derivatization | No derivatization |
| HPTLC Chromatograms |
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| Sample preparation | Macerate 500 mg of sample with 25 mL of chloroform for 15 minutes and filter. Evaporate the filtrate until dryness and dissolve the residue in 2 mL of chloroform |
| Mobile phase | n-hexane, ethyl acetate 7:3 (v/v) |
| Derivatization | Spray with modified Dragendorff reagent |
| Sample preparation | Macerate 500 mg of sample with 25 mL of chloroform for 15 minutes and filter. Evaporate the filtrate until dryness and dissolve the residue in 2 mL of chloroform |
| Mobile phase | n-hexane, ethyl acetate 7:3 (v/v) |
| Derivatization | Spray with anisaldehyde reagent, and heat the plate at 100°C for 3 min |
| HPTLC Chromatograms |
|
Related herbal drug(s)
Piper cubeba L. f.
Piper longum L.
Piper nigrum L.
Piper kadsura (Choisy) Ohwi (under development)
| Sample preparation | extract 4 g powdered drug with 100 mL of chloroform in a soxhlet apparatus for 4 hours. Filter and concentrate the extract under reduced pressure to 10 mL. |
| Mobile phase | n-hexane, acetone 7:3 (v/v) |
| Derivatization | No derivatization |